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mecp2 gfp rescue  (Addgene inc)


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    Addgene inc mecp2 gfp rescue
    Mecp2 Gfp Rescue, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mecp2+gfp+rescue/pEGFP-N1_MeCP2(WT)+(Plasmid+%23110186)/pm37712894-81-1-13
    Average 93 stars, based on 9 article reviews
    mecp2 gfp rescue - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants.
    Article Snippet: Genes mutated in monogenic neurodevelopmental disorders are broadly expressed.. This observation supports the concept that monogenic neurodevelopmental disorders are systemic diseases that profoundly impact neurodevelopment.. We tested the systemic disease model focusing on Rett syndrome, which is caused by mutations in MECP2.

    Article Title: Systemic Proteome Phenotypes Reveal Defective Metabolic Flexibility in Mecp2 Mutants
    Article Snippet: MECP2 knock out HAP1 cells from Horizon Discovery (Cat: HZGHC001102c010, RRID: CVCL_SX72) were cultured in IMDM ((Lonza, 12-722F) supplemented with 10% FBS (VWR 97068-085) in a humidified, 37°C, 10% CO 2 incubator. .. For MECP2-GFP rescue of HAP1 cells, pEGFP-N1_MeCP2(WT) was a gift from Adrian Bird (Addgene plasmid #110186; RRID:Addgene_110186), GFP only vector was created by digesting pEGFP-N1_MeCP2(WT) with BamHI and BglII to remove the MECP2 insert and ligated with T4 DNA ligase. .. Plasmids were transfected to HAP1 cells using FUGene according to manufactures protocol in Optimem Media (Gibco 31985070).

    Article Title: Systemic Metabolic and Mitochondrial Defects in Rett Syndrome Models
    Article Snippet: MECP2 knock out HAP1 cells from Horizon Discovery (Cat: HZGHC001102c010, RRID: CVCL_SX72) were cultured in IMDM ((Lonza, 12-722F) supplemented with 10% FBS (VWR 97068-085) in a humidified, 37°C, 10% CO 2 incubator. .. For MECP2-GFP rescue of HAP1 cells, pEGFP-N1_MeCP2(WT) was a gift from Adrian Bird (Addgene plasmid #110186; RRID:Addgene_110186), GFP only vector was created by digesting pEGFP-N1_MeCP2(WT) with BamHI and BglII to remove the MECP2 insert and ligated with T4 DNA ligase. .. Plasmids were transfected to HAP1 cells using FUGene according to manufactures protocol in Optimem Media (Gibco 31985070).



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    mecp2 gfp rescue  (Addgene inc)
    93
    Addgene inc mecp2 gfp rescue
    Mecp2 Gfp Rescue, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mecp2+gfp+rescue/pEGFP-N1_MeCP2(WT)+(Plasmid+%23110186)/pm37712894-81-1-13
    Average 93 stars, based on 1 article reviews
    mecp2 gfp rescue - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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